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immunoprecipitation method

См. также в других словарях:

  • Immunoprecipitation — (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many… …   Wikipedia

  • Chromatin immunoprecipitation — The procedure of chromatin immunoprecipitation (ChIP) assay and methods of analysis Chromatin Immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA in the… …   Wikipedia

  • Methylated DNA immunoprecipitation — (MeDIP or mDIP) is a large scale (chromosome or genome wide) technique that is used to enrich for methylated DNA sequences. It consists of isolating methylated DNA fragments via an antibody raised against 5 methylcytosine (5mC). This technique… …   Wikipedia

  • Protein-protein interaction — Protein protein interactions refer to the association of protein molecules and the study of these associations from the perspective of biochemistry, signal transduction and networks. The interactions between proteins are important for many… …   Wikipedia

  • Methods to investigate protein–protein interactions — There are many methods to investigate protein–protein interactions. Each of the approaches has its own strengths and weaknesses, especially with regard to the sensitivity and specificity of the method. A high sensitivity means that many of the… …   Wikipedia

  • ChIP-on-chip — Workflow overview of a ChIP on chip experiment. Contents …   Wikipedia

  • Chip-Sequencing — ChIP Sequencing, also known as ChIP Seq, is used to analyze protein interactions with DNA. ChIP Seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the cistrome of DNA associated proteins. It can… …   Wikipedia

  • Protein-protein interaction screening — Screening of Protein protein interactions refer to identify protein interactions with high throughput screening methods like computer and or robot assisted plate reading, flow cytometry analyzing etc. The interactions between proteins are of… …   Wikipedia

  • ChIA-PET — Chromatin Interaction Analysis by Paired End Tag Sequencing (ChIA PET) is a technique that incorporates chromatin immunoprecipitation (ChIP) based enrichment, chromatin proximity ligation, Paired End Tags, and ultra high throughput sequencing to… …   Wikipedia

  • Gel electrophoresis — apparatus – An agarose gel is placed in this buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. Classification… …   Wikipedia

  • Protein purification — is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. The starting material is… …   Wikipedia

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